In 1444 it was occupied by the Poor Clares at the express wish of Queen Maria, wife of Alfonso the Magnanimous, who wanted to have a place of retreat close to the city, and to this end she showered it with donations and privileges. The queen, who lived with her ladies in the neighbouring Royal Palace, had her own rooms in the monastery, where her tomb is also located. The Infanta Maria of Castile, wife of Alfonso the Magnanimous, King of Aragon, is also buried there, which is why she would have been known as Maria of Aragon, Viceroy of Valencia. Its library consists of around fifty parchments, documents and books of great value, for example, the autograph manuscript of the Vita Christi by Sister Isabel de Villena, the main abbess of the monastery; there is also one of the first editions of this work. There are several manuscripts by Jaume Roig, author of L'Espill, who was the nuns' doctor and one of those who contributed money for the construction of the monastery, several 15th century songbooks, a first edition of the Historia del convento de la Trinidad by Agustí Sales, the bull of Pope Leo X and the Libro de la casa de la Reina, a kind of inventory of the great families of 15th century Valencia and the alms they contributed to the construction of the monastery.

The cloister and the refectory are in the Valencian Gothic style and the church is Baroque in decoration. There are inscriptions on the keystones of the vault of the refectory and a Gothic double round arch that turns on itself. The "compass" or second cloister is a porticoed square from which the church is accessed. The façade of the church is a magnificent example of flamboyant Gothic and its decoration is reminiscent of the Lonja, and there is a replica of a magnificent Renaissance ceramic rose window. There are also several Baroque paintings and numerous relics, including a fragment of Christ's cross or lignum crucis and a thorn from his crown.

The convent is currently uninhabited, following the departure of the last cloistered nuns at the beginning of 2014, and can only be visited on request. You can admire the façade of the church, built in Valencian Gothic style with ashlar masonry and rammed earth.

The main door dates from the 15th century and is related to those of the Lonja. Above it, it is worth mentioning the beautiful Renaissance ceramic tondo, made in Italy, the original of which is kept in the National Ceramics Museum.